Effect of immersion time and kinetin growth regulator on micro tuber formation for Spunta potato.
DOI:
https://doi.org/10.37375/sjfssu.v5i2.3253الكلمات المفتاحية:
Solanum tubersum, growth regulators. temporary immersion system,microtubers.الملخص
This study was conducted at plant Tissue culture department which, belongs to Biotechnology research center located in Tripoli . In this study, micro tubers of Spunta potato variety were obtained through use of liquid culture medium by using temporary immersion system (RITA). The effect of liquid medium immersion time on plant growth was studied, as well as the effect of adding the kinetin growth regulator at 5 mg / l with 8% sugar on number and weight of formed tubers was also studied. The results showed that increase of immersion time from 1 minute to 2.5 minutes has a significant effect on increasing number and weight of formed tubers in each treatment. The average of number of tubers increased from 8.42 to 17.14 and 21.14 tuber in the vessel respectively. The total mean number of tubers in each pot was also increased when the kinetin growth regulator was added from 20.28 to 28.85 and 32.71 tuber per pot respectively. The average of tubers weight in each jar was also increased from 4.10 to 9.92g when the kinetin growth regulator was added.
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