In vitro phytochemical screening, Antioxidant and Anti-platelet Activities of Libyan Arbutus pavarii Extract
Keywords:Arbutus pavarii, Antioxidant, Antiplatelet.
This study was conducted to investigate the active compound like alkaloid and phenolic compound in leaves and fruit extracts of Arbutus pavarii compound and to determine the potential effect of methanol extract of Arbutus pavarii to improve antioxidant properties activity in a dose-dependent manner with good IC50 values. The antioxidant effect of Arbutus pavarii was investigated by scavenger effect using 1, 1-diphenyl-2 picrylhydrazyl (DPPH). And we observe the possibility of Arbutus pavarii extract to prevent human platelets aggregation. The effect of Arbutus pavarii extract related to its active compounds including polyphenols which able to reduce the scavenge of the hydroxyl radicals in human body leading prevent dysfunction pathway, the mechanism underlying such effects related to a reduce the vascular formation of Reactive oxygen species (ROS). The present findings indicated that Arbutus pavarii has antioxidant effects and the ability to prevent human platelets aggregation. These results could be indicator to improve the verity cells dysfunction in human body, then improve the function of cardiovascular and also to reduce pathological arterial wall. Further suggest that Arbutus pavarii might be an interesting candidate as a good food therapeutic.
Abdelwahed A, Bouhlel I, Skandrani I, Valenti K, Kadri M, Guiraud P, Steiman R, Mariotte AM, Ghedira K, Laporte F, Dijoux-Franca MG, Chekir-Ghedira L (2007). Study of antimutagenic and antioxidant activities of Gallic acid and 1,2,3,4, 6-ntagalloylglucose from Pistacialentiscus Confirmation by microarray expression profiling. Chemico-Biological Interactions 165: 1–13.
Auger a, Schini-Kerth . 2010. Nutritional improvement of the endothelial control of vascular tone by polyphenols: role of NO and EDHF.
Baratto MC, Tattini M, Galardi C, Pinelli P, Romani A, Visioli F, Basosi R, Pogni R (2003). Antioxidant activity of galloylquinic derivatives isolated from Pistacia lemniscus leaves. Free Rad. Res. 37(4): 405-412.
Benhammou N, AtikBekkara F, KadifkovaPanovska T (2007). Antiradical capacity of the phenolic compounds of Pistacialentiscus L and PistaciaatlanticaDesf. Adv Food Sci, 29(3): 155-16.
Born,G.V.R.(1971).PlateletaggregationandcyclicAMP.In: Effects of Drugson Cellular ContrMechanisms.Macmillan: London, in the Press. BORN, G.V.R. &DAWSON, H.A.(1971).
Corder R1, Mullen W, Khan NQ, Marks SC, Wood EG, Carrier MJ, Crozier A2006. red wine procyanidins and vascular health .Oncology.
Di Castelnuovo, A., Rotondo, S., Iacoviello, L., Donati, M.B., and De Gaetano, G. (2002).Meta-analysis of wine and beer consumption in relation to vascular risk.Circulation 105, 2836-2844.
Ehsani- Moghaddam, B Khanizadeh, S.,. and Levasseur, A. 2006. Antioxidant capacity in June-bearing and day-neutralstrawberry. Can. J. Plant Sci. 86 : 1387–1390.
El shatshat, S., (1997) Physiological Ecology ofArbutuspavariiPamp. M.Sc. Thesis, GaryounisUniversity, Benghazi, Libya.
El shatshat, S., (2009), Biological conservation ofthe endemic Arbutus pavariiPamp.: Seedgermination as attempt, International Journal OfHuman Geography And Environmental Studies.
Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. Lancet 1993; 341:454–7.red wine. Lancet 1993; 341:454–7.
Gardeli C, Papageorgiou V, Mallouchos A, Theodosis K, Komaitis M (2007). Essential oil composition of Pistacialentiscus L. and Myrtuscommunis L.: Evaluation of antioxidant capacity of metabolic extracts. Food Chem. pp. 1-11.
Kuriyama, S., Shimazu, T., Ohmori, K., Kikuchi, N., Nakaya, N., Nishino, Y., Tsubono, Y., and Tsuji, I. (2006). Green tea consumption and mortality due to cardiovascular disease, cancer, and all causes in Japan: the Ohsaki study. JAMA 296, 1255-1265.
Lee, J.O., Oak, M.H., Kang, S.H., Sohn, J.D., Kim, J.H., Lim, J., NA, Y., Oh, Y., and Lee, S.W.(2011). Composition for improving blood circulation, containing extract of Linder aobtusiloba as active ingredient, PCT, ed.
Madeira, S.V., Auger, C., Anselm, E., Chataigneau, M., Chataigneau, T., Soares de MouraR., And Schini-Kerth, V.B. (2009). E NOS activation induced by a polyphenol-richgrape skin extract in porcine coronary arteries. J VascRes 46, 406-416.
Mosmann Tim. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Meth. 1983;65(1–2):55–63.
Ndiaye, M., Chataigneau, M., Lobysheva, I., Chataigneau, T., and Schini-Kerth, V.B. (2005). Red wine polyphenol-induced, endothelium-dependent NO-mediated relaxation is dueto the redox-sensitive PI3-kinase/Akt-dependent phosphorylation of endothelial NOsynthasein the isolated porcine coronary artery. FASEB J 19, 455-457.
Ndiaye, M., Chataigneau, T., Chataigneau, M., and Schini-Kerth, V.B. (2003). Redwine polyphenolsinduce EDHF-mediated relaxations in porcine coronaryarteriesthrough the Redox-sensitive activation of the PI3-kinase/Aktpathway. Br J Pharmacol142, 1131-1136.
Renaud, S., and de Lorgeril, M. (1992). Wine, alcohol, platelets, and the French paradox forcoronary heart disease. Lancet 339, 1523-1526.
Sarr, M., Chataigneau, M., Martins, S., Schott, C., El Bedoui, J., Oak, M.H., Muller, B.,Chataigneau, T., and Schini-Kerth, V.B. (2006). Red wine polyphenols prevent angiotensinII-induced hypertension and endothelial dysfunction in rats: role of NADPH oxidase. Cardiovascular Res 71, 794-802.
Sofowora, A., in Medicinal Plants and Traditional Medicinal in Africa, Spectrum Books Ltd, Sunshine House, Ibadan, Nigeria, 1993, pp. 134-156.
Trease, G. E, W. C. Evans, Pharmacognosy, Saunders Publishers, London, 2002, pp. 42- 393.
How to Cite
Copyright (c) 2023 Scientific Journal for Faculty of Science-Sirte University
This work is licensed under a Creative Commons Attribution 4.0 International License.