Assessment of Allelochemicals Potential of Peganum harmala L. for Biological Control of Cynodon dactylon L.


  • Amal Ehtaiwesh Plant science Department, Science Faculty, Zawia University, Libya
  • Latifa Yarboa Veterinary Medicine and Agricultural Sciences Faculty, Zawia University, Libya



Cynodon dactylon L, Peganum harmala L, Herbicidal, Biological control.


This study was conducted to evaluates the allelopathic potential of Peganum harmala L. (Zygophyllaceae) on the growth of Cynodon dactylon L. (Poaceae). Seeds, stems, and a mixture of (seeds and stems) aqueous extracts of P. harmala plants were applied as a foliar application to three weeks old seedlings of Cynodon dactylon weeds. The result indicated that the application of aqueous extracts of different plant parts of P. harmala caused a significant reduction in growth traits of Cynodon dactylon including plant height, number of branches, number of leaves, and plant weight. Moreover, the result revealed that the Inhibitory effect of P. harmala aqueous extract on Cynodon dactylon L was more pronounced in plant height and number of leave plant-1 traits. In addition, the results illustrated that among the different P. harmala plant parts used in this study, seeds extract was the most toxic and caused the greatest negative effect on growth traits of Cynodon dactylon. According to the results, the degree of toxicity of different P. harmala plant parts can be arranged in the following order: seeds > mixture > stems. The results of this study concluded that the use of Peganum harmala L plant has the potential to be developed further as a bio-herbicide system to control weed such as Cynodon dactylon L.


Abu-Romman, S., Shatnawi, M., & Shibli, R. (2010). Allelopathic effects of spurge (Euphorbia hierosolymitana) on wheat (Triticum durum). American-Eurasian Journal of Agriculture and Environmental Science, 7(3), 298-302.

Aslam, M. M., Jamil, M., Malook, I., Khatoon, A., Rehman, A., Rahim, A., ... & Ul Bashar, K. (2016). Phytotoxic effects of Calotropis procera, Tamarix aphylla and Peganum harmala on plant growth of wheat and mustard. Pakistan Journal of Agricultural Research, 29(1).

Ashrafi, Z. Y., Sadeghi, S., Mashhadi, H. R., & Hassan, M. A. (2008). Allelopathic effects of sunflower (Helianthus annuus) on germination and growth of wild barley (Hordeum spontaneum). Journal of Agricultural Technology, 4(1), 219-229.

Batish, D. R., Arora, K., Singh, H. P., & Kohli, R. K. (2007). Potential utilization of dried powder of Tagetes minuta as a natural herbicide for managing rice weeds. Crop Protection, 26(4), 566-571.

Chatterjee, A., & Ganguly, M. (1968). Alkaloidal constituents of Peganum harmala L and synthesis of the minor alkaloid deoxyvascinone. Phytochemistry. 7, 307–311.

Dayan, F. E., & Duke, S. O. (2006). Clues in the search for new herbicides. In Allelopathy (pp. 63-83). Springer, Dordrecht.

Ehtaiwesh, A. (2021). Evaluation of herbicidal potential of Eucalyptus spp for biological control of Dactyloctenium aegyptium L. Journal of Pure & Applied Sciences. 20 (1): 186-191.

Farouk, L., A. Laroubi, R. Aboufatima, A. Benharref, & A. Chait, (2008). Evaluation of the analgesic effect of alkaloid extract of Peganum harmala L.: Possible mechanisms involved, Journal of Ethnopharmacology 115. 449-454.

Hasanuzzaman, M., Ali, M. H., Alam, M. M., Akther, M., & Alam, K. F. (2009). Evaluation of pre-emergence herbicide and hand weeding on the weed control efficiency and performance of transplanted Aus rice. American-Eurasian Journal of Agronomy, 2(3), 138-143.

Hong, N. H., Xuan, T. D., Eiji, T., & Khanh, T. D. (2004). Paddy weed control by higher plants from Southeast Asia. Crop Protection, 23(3), 255-261.

Hussain, S., Siddiqui, S. U., Khalid, S., Jamal, A., Qayyum, A., & Ahmad, Z. (2007). Allelopathic potential of senna (Cassia angustifolia Vahl.) on germination and seedling characters of some major cereal crops and their associated grassy weeds. Pakistan Journal of Botany, 39(4), 1145.

Imran, A. M. (2014). Effect of Peganum harmala L. on seed germination, seedling growth and Calcium Potassium content of Triticum aestivum and Hordeum Vulgare. Journal of University of Babylon, 22(9), 2451-2444.

Islam, A. K. M. M., & Kato-Noguchi, H. (2012). Allelopathic potentiality of medicinal plant Leucas aspera. International Journal of Sustainable Agriculture, 4(1), 01-07.

Kartal, M.; Altun, M.L.& Kurucu, S. (2003). HPLC method for the analysis of harmol, harmalol, harmin and harmaline in the seeds of Peganum harmala L. J. Pharm. Biomed. Anal. 31, 263–269.

Khan, A. M., Qureshi, R. A., Ullah, F., & Gilani, S. A. (2011). Phytotoxic effects of selected medicinal plants collected from Margalla Hills, Islamabad Pakistan. Journal of Medicinal Plants Research, 5(18), 4671-4675.

Khan, R., Basit, A., Rasheed, S. M., & Shah, S. S. (2020). Allelopathic effects of plant parts of two invasive weeds on seed germination and seedling growth of open pollinated Maize varieties. Pakistan Journal of Weed Science Research, 26(1), 83.

Khanh, T. D., Xuan, T. D., Hahn, S. J., & Chung, I. M. (2004). Methods to screen allelopathic accessions of wheat, barley, oat, sorghum and cucumber for weed control. Allelopathy Journal, 14(2), 145-165.

Macías, F. A., Chinchilla, N., Varela, R. M., & Molinillo, J. M. (2006). Bioactive steroids from Oryza sativa L. Steroids, 71(7), 603-608.

Mahmoudian, M., H. Jalilpour, & P. Salehian, (2002). Toxicity of Peganum harmala L: review and a case report, Iranian Journal of Pharmacology & The rapectics 1. 1-4.

Movafeghi, A., Abedini, M., Fathiazad, F., Aliasgharpour, M., & Omidi, Y. (2009). Floral nectar composition of Peganum harmala L. Natural product research, 23(3), 301-308.

Mubeen, K., Nadeem, M. A., Tanveer, A., & Zahir, Z. A. (2011). Allelopathic effect of aqueous extracts of weeds on the germination and seedling growth of rice (Oryza sativa L.). Pak. J. life soc. Sci, 9(1), 7-12.

Ninsuwan, N., Chuenchit, S., & Petcharat, V. (2011). Evaluation of Exserohilum longirostratum (Subram.) Sivan. for biological control of Dactyloctenium aegyptium (L.) Beauv. Journal of Agricultural Technology, 7(2), 463-473.

Poonpaiboonpipat, T., Krumsri, R., & Kato-Noguchi, H. (2021). Allelopathic and Herbicidal Effects of Crude Extract from Chromolaena odorata (L.) RM King and H. Rob. on Echinochloa crus-galli and Amaranthus viridis. Plants, 10(8), 1609.

Shanna, K. D., Sidana, K. L. & Singhvi, N. R. (1982). Allelochemic effect of Peganum harmala L. on Pennisetum typhoideum. Indian Journal of Botany S: 115-119.

Shao, H., Huang, X., Zhang, Y., & Zhang, C. (2013). Main alkaloids of Peganum harmala L. and their different effects on dicot and monocot crops. Molecules, 18(3), 2623-2634.

Sharaf, M.; El-Ansari, M.A.; Matlin, S.A.; & Saleh, N.A.M. (1997). Four flavonoid glycosides from Peganum harmala L. Phytochemistry, 44, 533–536.

Shemdoe, R. S., Mbago, F. M., Kikula, I. S., & Van Damme, P. L. (2008). Weed species diversity on arable land of the dryland areas of central Tanzania: Impacts of continuous application of traditional tillage practices. GeoJournal, 71(2-3), 107-115.

Singh, H. P., Batish, D. R., Kaur, S., & Kohli, R. K. (2003). Phytotoxic interference of Ageratum conyzoides with wheat (Triticum aestivum). Journal of agronomy and crop science, 189(5), 341-346.

Sodaeizadeh, H., Rafieiolhossaini, M., Havlík, J., & Van Damme, P. (2009). Allelopathic activity of different plant parts of Peganum harmala L. and identification of their growth inhibitors substances. Plant Growth Regulation, 59(3), 227.

Turk, M. A., & Tawaha, A. M. (2003). Allelopathic effect of black mustard (Brassica nigra L.) on germination and growth of wild oat (Avena fatua L.). Crop protection, 22(4), 673-677.

Xuan, T. D., Shinkichi, T., Khanh, T. D., & Chung, I. M. (2005). Biological control of weeds and plant pathogens in paddy rice by exploiting plant allelopathy: an overview. Crop protection, 24(3), 197-206.

Xuan, T. D., Shinkichi, T., Hong, N. H., Khanh, T. D., & Min, C. I. (2004). Assessment of phytotoxic action of Ageratum conyzoides L. (billy goat weed) on weeds. Crop protection, 23(10), 915-922.

Zhao, T., Wang, Z. T., Branford‐White, C. J., Xu, H., & Wang, C. H. (2011). Classification and differentiation of the genus Peganum indigenous to China based on chloroplast trnL‐F and psbA‐trnH sequences and seed coat morphology. Plant Biology, 13(6), 940-947.




How to Cite

Ehtaiwesh, A., & Yarboa, L. (2021). Assessment of Allelochemicals Potential of Peganum harmala L. for Biological Control of Cynodon dactylon L. Scientific Journal for Faculty of Science-Sirte University, 1(2), 1–6.