Assessment of Juniperus Phoenicea L. Seedling Produced via Tissue Culture Using RAPD Analysis
DOI:
https://doi.org/10.37375/sjfssu.v5i1.3207Keywords:
Dormancy, Juniperus L., plant regeneration; , Proliferation, RAPD analysisAbstract
Woody plants are more difficult to reproduce than other plant species found in nature. In addition to seed dormancy, which is caused by inhibitors in the embryo, endosperm, and/or testicle, Phoenicia, like other juniper species, does not exhibit a high rate of plant production by germination of seeds.
This research's primary goals were to propagate Juniperus phoenicea L. (J. phoenicea) using tissue culture by identifying a promising protocol for utilizing kinetin (0.5 mg/l-1 with Murashige and Skoog 1962, MS), Rugini Olive Medium 1984, ROM, and Lloyd and McCown 1980, WPM to break the secondary dormancy of seeds. Additionally, RAPD fingerprints were used for molecular analysis to determine the total genomic DNA of the parent plant compared to the in vitro plant obtained.
The RAPD-PCR products they obtained demonstrated a high percentage of polymorphism resulting from using BA-K5 as a primer compared to BA-K2. The results showed that the medium (WPM) supplemented with 0.5 g/l⁻¹ kin gave the highest significant increment for germination percentage (60%), while MS gave the lowest germination rate (8%). The study concludes that the in vitro culture method for zygotic embryo germination may be a viable way to overcome the challenges posed by poor juniper germination rates and boost seed propagation
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