Parasite Contamination of Fresh Leafy Vegetables in Benghazi, Libya.

Authors

  • Entesar S. Elmnefi Department Of Medical Technology. College of Sciences and Technology* Gaminis , Benghazi , Libya.
  • Najat I. Alshibli 1Department Of Medical Technology. College of Sciences and Technology* Gaminis , Benghazi , Libya
  • Najwa M. Alfurjani Department Of Medical Technology. College of Sciences and Technology* Gaminis , Benghazi , Libya
  • Heba A. Salh Department Of Medical Technology. College of Sciences and Technology* Gaminis , Benghazi , Libya.
  • Sami F. Altom Depertment Parasitology, Referencial Medical laboratory of Benghazi, Benghazi, Libya.
  • Mohammad A. Faraj Department Of Medical Technology. College of Sciences and Technology* Gaminis , Benghazi , Libya.
  • Fawzia A. Al- Majbri Depertment Parasitology, Referencial Medical laboratory of Benghazi, Benghazi, Libya

DOI:

https://doi.org/10.37375/sjfssu.v4i2.2893

Keywords:

Vegetables, Helminths, Parasitology, parasitic diseases, contamination, , Libya

Abstract

Background: Raw vegetable consumption can be a primary pathway for the spread of food-borne parasite illnesses, despite the fact that fresh vegetables offer significant nutritional value to people. Thus. This study's goal was to investigate whether parasites are present in veggies that are frequently eaten in Benghazi city, Libya. Method of the study:- One hundred and fifteen types of vegetables. lettuce, mint, parsley, green onion, and watercress 30 pieces of each were included. The sediments experienced analysis using iodine and modified smears stained with Ziehl- Neelsen, while data SPSS version 20 statistical software was used for the analysis. Results:- It was discovered that 90% of the samples that were inspected contained parasite eggs, cysts, and larvae. With a contamination percentage of 100%, lettuce was the most contaminated vegetable, and parsley had the lowest contamination rate (80%). The parasite with the highest prevalence rate was Entamoeba histolytica/ dispar cyst (71.1%), Giardia Lamblia cyst and Hymenolepis nana eggs (13.3% each of them), Taenia. Saginata eggs (11.11%), Ascaris eggs and Entamoeba. Hartmanni cyst (6.67% each of them), but it was discovered that the Strongyloides larvae only occasionally appeared (6.67%).

Conclusion:- Benghazi City leafy vegetables had a very high parasitic rate of contamination. Therefore, eating such vegetables without thoroughly cleaning them promotes the spread of parasitic diseases, which provide a serious risk to health for those in Libya.

References

Al-Binali A. M, Bello C.S, El-Shewy K, Abdulla S.E. The prevalence of parasites in commonly used leafy vegetables in South Western, Saudi Arabia. Saudi Medical Journal. (2006), Vol 27: 613- 616.

Al-Shawa RM, Mwafy S. N. The enteroparasitic contamination of commercial vegetables in Gaza Governorates. J Infect Dev Ctries, (2007), vol 1(1):62-66.

Abougrain A. K, Nahaisi M. H, Madi N. S, Saied M. M, and Ghenghesh K. S, Parasitological contamination in salad vegetables in Tripoli-Libya, Food Control, (2010), vol. 21, no. 5, pp. 760– 762.

Al-Megrain W. A. I. Prevalence of intestinal parasites in leafy vegetables in Riyadh, Saudi Arabia. International Journal of Zoological Research, (2010), vol 6(3):190-195.

Ammar, A. S. & Omar, H. M. The prevalence of leafy vegetable- borne parasites in Al-Qasslm region, Saudi Arabia. Journal of Agricultural and Veterinary Sciences, (2012), vol 6(1) doi:10.12816/0009444.

Adejayan. A, Morenikeji O, Prevalence of Intestinal parasites in vegetables sold in major markets in Ibadan city, south-west Nigeria. Global journal of pure and applied sciences. (2015), vol 21:7- 12.

AL-Sanabani, A. W, Algalil, F. M.A, Radman, B. A. & AL-Manusorl, R. T Prevalence of intestinal parasites in fresh leafy vegetables in some farms at Dhamar city, Yemen. Prevalence, (2016).

Alegbeleye O. O, Singleton I, Sant’Ana A. S, Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review. Food Microbiol. Google Scholar [CrossRef]. (2018), vol 73, 177–208.

Ahmed A.A, Sayed F.G, Galal L.A, Ismail T, Gaber M.M, Detection of parasites contaminating raw consumable vegetables in Assiut city, Assiut governorate, Egypt. Journal of the Egyptian Society of Parasitology, (2020), vol 50: 557-564.

Al-Niaeemi1. B H, Ahmed1. N. M, Kharofa. W A, Parasitic contamination of some fresh and collected vegetables from Mosul City markets, Revis Bionatura, (2022), Vol 7(3) 26.

Beuchat LR, Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables, Microbes Infect. (2002), vol 4(4):413-23. PubMed.

Budu-A. E, Greenwood SJ, Dixon BR, Barkema HW, McClure J. T. Occurrence of Cryptosporidium and Giardia on beef farms and water sources within the vicinity of the farms on Prince Edward Island, Canada. Vet Parasitol;( 2012), vol 184(1):1-9.

Bekele F, Tefera T, Biresaw G., Yohannes T. Parasitic contamination of raw vegetables and fruits collected from selected local markets in Arba Minch town, southern Ethiopia. Infectious Diseases of Poverty. (2017), vol 6: 19.

Cheesbrough O. & Arthur O. A. laboratory manual for rural tropical Hospitals. Basis for training courses. Churchill Livingstone, Edinburgh. (1976), vol 209 pp.

CDC. National outbreak reporting system available online,https://www.cdc.gov/foodsafety/communication/leafy-greens.html. (2022), (accessed on 24 July).

Dorris, M. Viney, E. and Blaxter, M. Molecular phylogenetic analysis of the genus Strongyloides and related nematodes. Internatinal Journal of Parasitology, (2002), vol 32(12): 1507-1517.

Damen J. G, Banwat E. B, Egah D. Z., and Allanana J. A, Parasitic contamination of vegetables in Jos, Nigeria, Annals of African Medicine, (2007), vol 6, no 3, pp 115–118.

Daryani, A. Ettehad, G.H. Sharif, M. Ghorbani, L. and Ziaei, H. “Prevalence of intestinal parasites in vegetables consumed in Ardabil, Iran,” Food Control, (2008) vol. 19, no. 8, pp. 790–794,

Dias M, George M, and Dias M.R. How safe are vegetables parasitological contamination of fresh leafy vegetables in Mangalore, International Journal of Applied Biology and Pharmaceutical Technology, (2014), vol 5(4): 80-82.

Duedu K.O, Yarnie E.A, Tetteh-Quarcoo P.B, Attah S.K, Donkor E.S, Ayeh-Kumi P.F. A comparative survey of the prevalence of human parasites found in fresh vegetables sold in supermarkets and open-aired markets in Accra, Ghana. BMC Res Notes (2014), vol 7:836.

Erdogrul O, Sener H. The contamination of various fruit and vegetables with Enterobius vermicularis, Ascaris eggs, Entamoeba histolytica cysts and Giardia lamblia cysts. Journal Food control, (2005), vol 16:527-560.

Ebrahimzadeh. A, Jamshidi. A, & Mohammadi. S, The parasitic contamination of raw vegetables consumed: Zahedan, Iran. Health Scope, (2013), vol 1(4): 205-209 Doi: 10.5812 /jhs.8209.

Ezatpour B, Chegeni A.S, Abdollahpour F, Aazami M, Alirezaei M. Prevalence of parasitic contamination of raw vegetables in Khorramabad, Iran. Food control, ( 2013) vol 34:92-95.

Elmajdoub L.O, Omar M.A, Al-Aboudy M.S, Abosheba F, Elzwawi S, Prevalence of parasitic contamination of leafy green vegetables in Misurata, Libya Russian Journal of parasitology. (2017), vol 40: 197- 204.

Etewa S.E, Abdel-Rahman S.A, Fathy G.M, Abo El-Maaty D.A, Sarhan M.H. Parasitic contamination of commonly consumed fresh vegetables and fruits in some rural areas of Sharkyia government, Egypt. Afro-Egyptian Journal of Infectious and Endemic Diseases, (2017), vol 7: 192-202. [DOI: 10. 21608/AEJI.2017.17804].

El-Sayed. N. M, Gawdat, S. S, El-Kholy. H.S, Elmosalamy. A. Parasitic Contamination in Five Leafy Vegetables Collected from Open Market places in Giza, Egypt, Journal of Food Quality and Hazards Control 10 (2023), vol 13-20.

Gharavi M. J, Jahani M. R, and Rokni M. B, Parasitic contamination of vegetables from farms and markets in Tehran, Iranian Journal of Public Health, (2002), vol 31, no 4, pp 83–86.

Garedaghi Y, Hashemzade F, & Pooryagoobi S. Parasitic contamination of fresh Vegetables consumed in Tabriz,Iran. Reasearch Tournal of Biological Sciences. (2011), vol 6(10):518-522.

Hadi A. M, Isolation and identification of intestinal parasites from vegetables from different markets of Iraq, Bulletin of the Iraq Natural History Museum, (2011) vol. 11, no. 4, pp. 17–25.

Halablab M.A, Sheet I.H, Holail H.M, Microbiological Quality of Raw Vegetables Grown in Bekaa Valley Lebanon. Am. Journal Food Technol. [Google Scholar] [CrossRef][Green Version]. (2011), Vol 6, 129–139.

Hassan A, Farouk H, and Abdul R -Ghani, Parasitological contamination of freshly eaten vegetables collected from local markets in Alexandria, Egypt: a preliminary study, Food Control, (2012), vol. 26, no. 2, pp. 500–503.

https://earth.google .com.

https:// images. App. Google /trWn367XXv5odCg.

Iwu C.D, Okoh A.I. Preharvest Transmission Routes of Fresh Produce Associated Bacterial Pathogens with Outbreak Potentials. A Review. Int. Journal Environ Res Public Health Google Scholar [Cross Ref][Green Version]. (2019), vol 16, 4407.

Kniel K.E, Lindsay D.S, Sumner S.S, Hackney C.R, Pierson M.D, and Dubey J.P. Examination of attachment and survival of Toxoplasma gondii oocysts on raspberries and blueberries. Journal parasitol, (2002), vol 88:790-793.

Kozan E, Sevimi F. K, Kose M, Eserm M, and Cicek H, Examination of helminth contaminated wastewaters used for agricultural purposes in Afyonkarahisar, Turkish Journal of Parasitology, (2007), vol. 31(3), pp. 197–200. PubMed.

Kudah C, Sovoe S and Baiden F. Parasitic contamination of commonly consumed vegetables in two markets in Ghana. Ghana Med J. (2018) Jun;52(2):88-93. PubMed.

LIU, R.H, Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals, The American Journal of Clinical Nutrition, (2003), vol 78, no. 3, pp.517S-520S, Doi PMid:12936943.

Mehraj, V. hatcher, J. Akhtar, S. Rafique, G. and Beg, M. A, Prevalence and factors associated with intestinal parasitic contamination among children in an urban Slum of Karachi. Plos, One, doi: journal pone.0003680, (201 3), vol (1l): 15-20.

Murray K, Wu, F, Shi J, Jun Xue, S, Warriner K. Challenges in the microbiological food safety of fresh produce: Limitations of post-harvest washing and the need for alternative interventions. Food Qual. Saf. (2017), Vol 1, 289–301.

Marie. c, Rahway c. o, Overview of parasitic infections. Merck NJ, USA. (2023).

Northrop. C. A & Shaw. C, Parasites Br. Med Bull. (2000), vol 56:193-208.

Nyarango R. M, Aloo P. A, Kabiru E. W, Nyanchongi B. O. The risk of pathogenic intestinal parasite infections in Kisii Municipality, Kenya. BMC public health. (2008) 8:237. PubMed.

Ogbolu D. O, Alli O. A, Ogunleye V. F, The presence of intestinal parasites in selected vegetables from open markets in south western Nigeria. Afr J Med Med Sci (2009) Dec;38(4):319-324

Oladele H. A, Ifeanyi. O. E, Akinleye C, Oluwanisola D. O, Public health risks associated with fruits and vegetables at Owo markets, International Journal of Current Research in Sciences. (2022), Vol 8: 29-41. [DOI: 10.22192/ijcrms.2022.08. 01.003]

Pires S. M, Vieira A. R, Perez E, Lo F, Wong D, Hald T. Attributing human food borne illness to food sources and water in Latin America and the Caribbean using data from outbreak investigations. Int J Food Microbial; (2012), vol 152(3):129 – 38.

Robertson L.J. et al. Isolation and enumeration of Giardia cysts, Cryptosporidium oocysts, and Ascaris eggs front fruits and vegetables. Journal of Food Protection. (2000).

Srikanth R, and Naik. D, Health effects of wastewater reuse for agriculture in the suburbs of Asmara City, Eritrea, International Journal of Occupational and Environmental Health, (2004), vol. 10, no. 3, pp. 284–288.

Shahnazi, M. and Jafari-Sabet, M. Prevalence of parasitic contamination of raw vegetables in villages of Qazvin province, Iran, Foodborne Pathogens and Disease, (2010) vol. 7, no. 9, pp. 1025–1030.

Said D. E, Detection of parasites in commonly consumed raw vegetables, Alexandria Journal of Medicine, (2012), vol. 48, pp. 345–352.

Sia Su G. L, Mariano C. M. R, Matti N. S. A, and Ramos G. B, Assessing parasitic infestation of vegetables in selected markets in Metro Manila, Philippines, Asian Pacific Journal of Tropical Disease, (2012), vol 2, no 1, pp 51–54.

Torrence M. E, Introduction to Preharvest Food Safety. Microbiol. Spectr. [Google Scholar] [CrossRef] [PubMed]. 2016. Vol 4.

Wikipedia H. M. Improvement of Ritchie technique by identifying the food that can be consumed pre-analysis. J App Sci Res, (2009), vol 5:293-296.

World Health Organization, Diet, Nutrition and the Prevention of Chronic Diseases. Report of a Joint FAO/WHO Expert Consultation, (2003), Vol. 916.

Youssef A. I, Uga S. Review of parasitic zoonoses in Egypt. Tropical Medicine and Health. (2014), 42: 3-14.

Zohour, A, and Molazadeh, P, Prevalence of pathogenic parasites in consumed vegetables in Jiruft, Journal of Birjand University of Medical Sciences, ( 2001) vol. 8, pp. 10–13.

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Published

2024-10-26

How to Cite

Elmnefi, E. S., Alshibli, N. I., Alfurjani, N. M., Salh, H. A., Altom, S. F., Faraj, M. A., & Al- Majbri, F. A. (2024). Parasite Contamination of Fresh Leafy Vegetables in Benghazi, Libya. Scientific Journal for Faculty of Science-Sirte University, 4(2), 62–72. https://doi.org/10.37375/sjfssu.v4i2.2893