Detection of MUTYH Gene Mutations in Hereditary Colorectal Cancer Libyan Families
Colorectal Cancer (CRC) has become one of the most dangerous yet spreadable cancers, as a result of many wrong behaviors people do, in addition to being a hereditary disease. However, Libya was not included in a lot of studies due to the lack of adequate studies on infected CRC Libyan patients, thus this study aims to detect mutations in the MUTYH gene in Libyan families who heredity colorectal cancer. The study included 20 blood samples collected from (10 patients with hereditary colorectal cancer and 10 healthy people) all of them had a family history of this disease. Genomic DNA was extracted, amplified by PCR, and analyzed for MUTYH mutational status by direct sequencing. MUTYH mutations were present in 50% (10\20) of all analyzed samples. A total of 10 patients had MUTYH mutations in different positions; of which 5\10 (50%) had a deletion A in c.1140, 1\10 (10%) a patient had a substitution mutation in c.1149 C>N, 1\10 (10%) a patient had two type mutations, substitution mutation in c.1154 C>T and insertion CT inc.1153, 2/10 (20%) they had substitution mutation in c.1237 G>R, 1\10 (10%) a patient had substitution mutation in c.1140 A>C. This study concludes that indicates that analysis of the MYH gene should be performed in patients with multiple colorectal adenomas, On the other hand, it helped to clarify the type and frequency of MYH mutations among colorectal polyposis patients in Misurata. This study believes that an enlargement of the MUTYH mutation spectrum resulting from these types of studies will contribute to early detection and the prevention of secondary cancer development.
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