Muco-adhesion Evaluation of Polysaccharides in Simulated Physiological Fluids
Keywords:Gellan gum, Methylene blue, Retention time, Gellan gel, Drug delivery.
Gellan gum is a microbial exopolysaccharide, water-soluble polymers secreted by microorganisms during fermentation. The biopolymer gellan gum is a relatively recent addition to the family of microbial polysaccharides that is gaining much importance in food, pharmaceutical and chemical industries due to its novel properties. The purpose of this work is to investigate the impact of physiological fluids on both the physical and chemical properties of gellan gum, and to understand the role of polymers gel in muco-adhesion and drug delivery to prolong the residence time of the drug inside the body. Muco-adhesion measurements of retention time were performed using bespoke retention apparatus to determine the retention of labelled gellan gum dose. The physiological fluids used in this work are artificial gastric juice, artificial saliva fluid, and artificial tears fluid. Results of this work show that in general the viscosity of gellan increased with high concentration and the gel formation is strong with artificial gastric juice (HCL) and weak gel formation with artificial saliva and tears but the retention time is longer with saliva and tears than with artificial gastric juice.
Batchelor, H. K., Banning. D., Dettmar, P.W., Hampson, F.C., Jolliffe, I. G., Craig, D. Q. M., (2002) An in vitro mucosal model for prediction of the bio-adhesion of alginate solutions to the oesophagus, International Journal of Pharmaceutics, 238: 1–2, 123-132.
Bernkop-Schnürch, A., & Steininger, S. (2000). Synthesis and characterisation of mucoadhesive thiolated polymers. International journal of pharmaceutics, 194(2), 239–247.
Duchene, D., Touchard F., Peppos, N.A., (1988). Drug Dev, 1nd Pharm, 14:283.
Gilbard, J. P., Human tear film electrolyte concentrations in health and dry-eye disease, Int Ophthalmol Clin. 1994, 34:27-36.
Glasgow, B. J, Gasymov, O. K., Abduragimov, A. R., Engle, J. J., Casey, R. C., (2010) Tear lipocalin captures exogenous lipid from abnormal corneal surfaces. Invest Ophthalmol Vis Sci. 51:1981-7.
Huang, Y., (2004). Gelling temperatures of high acyl gellan as affected by monovalent and divalent cations with dynamic rheological analysis, Carbohydr. Polym, 56, 27-33.
Humphrey, S. P., Williamson, R. T., (2001). A review of saliva: normal composition, flow, and function. The Journal of Prosthetic Dentistry, Vol. 85, 162.
Jansson, P. E., Lindberg, B., and Sandford, P. A. (1983) Structural studies of gellan gum, an extracellular polysaccharide elaborated by Pseudomonas elodea, Carbohydr. Res., 124, 135-139.
Kamath, K. R, Park, K., (1994). Mucosal adhesive preparations, in Encyclopaedia of Pharmaceutical Technology; J. Swarbrick and J. C. Boylan, eds., Vol. 10 Marcel Dekker, New York, 133.
Mao M., Tang J., Swanson B.G. (2000). Texture properties of high and low acyl mixed Gellan gels, Carbohydr, polmr., 41, 331-338.
Matricardi, P., Cencetti, C., Ria, R., Alhaique, F., and Coviello, T. (2009). Preparation and Characterization of Novel Gellan Gum Hydrogels Suitable for Modified Drug Release, Molecules, 14, 3376-3391.
Nagai T, Machida Y. (1985). Mucosal adhesive dosage forms, Pharm Int, 6: 196-200.
Sanderson, G. R., Clark, R. C., (1983). Gellan gum, Food Technology, 37, 62–70.
Stjernschantz, J., Astin, M., (1993). Anatomy and physiology of the eye physiological aspects of ocular drug therapy, In: Biopharmaceutics of Ocular Drug Delivery, Edman, P. ed., CRC Press, Boca Raton, pp. 1–25.
How to Cite
Copyright (c) 2022 Scientific Journal for Faculty of Science-Sirte University
This work is licensed under a Creative Commons Attribution 4.0 International License.