In vitro Micropropagation of Ginger plant (Zingiber officinale)

Authors

  • Ahmed Shaaban Libyan Biotechnology Research Center Department tissue culture plant
  • Noman Elnfishy Libyan Biotechnology Research Center Department tissue culture plant
  • Zineb Aween Libyan Biotechnology Research Center Department tissue culture plant
  • Elshabany Abdelah Libyan Biotechnology Research Center Department tissue culture plant
  • Elmundr Abughni Libyan Biotechnology Research Center Department tissue culture plant

DOI:

https://doi.org/10.37375/sjfssu.v3i2.1659

Keywords:

Micropropagation, Plant growth regulators, Tissue culture, Zingiber officinale

Abstract

The study was conducted for the purpose of micro propagation of ginger plant (Zingiber officinale) through use of plant tissue culture technique to identify the best plant micro propagation conditions. The plant samples were sterilized superficially by immersing them in the Clorox solution, and then the sterilized plants were cultured in MS media in order to obtain free contamination culture. After the sufficient number of ginger plants had obtained the plants were replanted in MS media supplemented with several concentrations of (BA and NAA) for the purpose of obtaining the best vegetative growths. The treatments were divided into four treatments as T1 (control treatment contains MS without hormones), T2 (MS +0.1mg/l NAA+1mg/l BA), T3 (MS+0.1mg/l NAA+2mg/l BA) and T4 (MS+ 1mg/l NAA). The results of this study showed that addition of 0.1mgNAA with 1 or 2mg/l BA improve the vegetative growth of ginger plant. It is also proved that the average of plant length, number of brunches and number of leaves was significantly higher in T2 and T3 compared with other two treatments. Furthermore, results proved that combination between NAA and BA gave the best vegetative growth. For the root site, the results showed that the culture media (MS) plus 1 mg / L of the growth regulator (NAA) resulted in the highest of root growth. A cording to our results addition 0.1mg NAA with 1 or 2mg/l BA would be the best choice for In vitro Micropropagation of ginger plan.

References

Behera K. K., Pani O and Sahoo S. (2010). Effect of plant growth regulator on in vitro multiplication of turmeric (Curmumalomga L. cv Ranga). Int. J. Biol. Technol., 1:16-23. bioassays with tobacco tissue cultures. Physiol Plant1 5:473 - 479. Constituents of Zingiber officinale rhizomes produced by in vitro shoot tip culture. culture. The Plymouth Student Scientist 4:432-449.

Dadi T L, Dejene T B and Hatabtamu G M. (2020). Micro propagation of medicinal plants. international journal of plant breeding and crop science. vol 7(2) pp. 796-802. Issn :2167.

Devina D, Teoh Y J and Jualang A G. (2016). I n vitro propagation of Zingiber officinal. Transactions science and technology. (1 – 2) ,162-167

Garcia-Gonzales R, Quiroz K, Carrasco B, Caligari P (2010) Plant tissue culture: Current status, opportunities and challenges. Cien. Inv. Agr. 37(3): 5-30.

Kambaska, K. B. and Santilata, S. (2009). Effect of Plant Growth Regulator on Micropropagation of Ginger (Zingiber officinale Rosc.). Journal of Agricultural Technology 5(2):271-280.

Kheiry K, Ahmed S, Elmundr A, Mhammed A, Salem H and Areje S. (2020). Micropropagation of Zingiber officinal Roscoe. journal of Misurata university for agriculture science 1:1 38-50. ISSN 2708: 8588.

Kumar S. and Singh, N. (2009). Micropropagation of Prosopis cineraria. Druce – amulti purposed esert tree. Researcher,1(3):9-13.

Kumari R. and Priya (2020). Optimization of Concentrations of Plant Growth Regulators For In Vitro Multiple Shoot Formation and Efficient Root Induction in PhylanodifloraL.(Lippia nodiflora L.) An Important Medicinal Plant. Indian J. Sci. Res.10(2):39-44. medicinal plants. Biotechnology Adv 18:91-

Murashige T, Skoog F (1962) A revised medium for the rapid growth and Murashinge T and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures plant1:473 –497.

Nayak, S. and Naik, P.K. (2006). Factors Effecting In Vitro Micro Rhizome Formation and Growth In Curcuma longaL. and Improved Field Performance of Micro Propagated Plants, ScienceAsia.32:31-37.

Newman, M. (2001). Nomenclatural notes on Zingiberaceae. Rapid clonal propagation of ginger (Zingiber officinales Rosc.). Japan J.Breed.38: 437-442.

Ravindran, P. N. & Nirmal Babu, K. (2005). Ginger the Genus Zingiber. United States of America: CRC Press. Tissue culture and biotechnology of Ginger. Photochemistry 25:1333

Rout, G.R., S.K. Palai, S. Samantaray and P. Das. (2001). Effect of growth regulator and culture conditions on shoot multiplication and rhizome formation in ginger (Zingiber officinale Rosc.). In vitro Cell. Dev. Biol.-Plant, 37: 814-819

Sarasan V., Kite, G. C., Sileshi, Gudeta. W. S. Philip C.S. (2011). Applications of photochemical and in vitro techniques for reducing over-harvesting of medicinal and pesticide plants and generating income for the rural poor. Plant Cell Rep 30, 1163–1172.https://doi.org/10.1007/s00299-011-1047-5.

Sharma K and Malik B. (2014). In vitro conservation of Zingier officinal through rhizome. journal of pharmacy and biological science. ISSN:2319-7676.Vol 9: pp39-40.

Sidhu Y (2010). In vitro Micropropagation of medicinal plants by tissue.

Downloads

Published

2023-10-26

How to Cite

Shaaban, A., Elnfishy, N., Aween, Z., Abdelah, E., & Abughni, E. (2023). In vitro Micropropagation of Ginger plant (Zingiber officinale). Scientific Journal for Faculty of Science-Sirte University, 3(2), 154–161. https://doi.org/10.37375/sjfssu.v3i2.1659

Issue

Vol. 3 No. 2 (2023): Volume 3 Issue No 2 2023

Section

BOTANY

License

Copyright (c) 2023 Scientific Journal for Faculty of Science-Sirte University

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.