The Antimicrobial Effect of The Artimisa Herba and Eordium Glycobhyllum Against A Few of Pathogenic Bacteria
Keywords:
Artimisa herba, Eoridum, Pathgenic bacteriaAbstract
Artimisa herba and Erodium antimicrobial activity were used against Serratia spp., Bacillus cereus, E.coli, Micrococcus roseus, Staphylococcus aureus, Streptomyces spp.and Micrococcus lutaus. The method of extraction applied in this paper was the Soxhlet- hot extraction using petroleum ether and alcoholic solvents. The bacterial inhibition by the extract was as follows:
Serratia spp was found moderately sensitive to Artimisa herba with failure to produce pigment. B. cereus was found moderately so to Artimisa herba lal, while it showed low response to Artimisa herba. Escherchia coli showed moderate inhibition to Artimisa herba, Micrococcus roseus responded well to and grodium moderately to grodium. Staohylococcus aureus well to moderately to Artimisa herba. Streptomyces spp. responded well only to be based on the results obtained in this study, it can be concluded that using 50% acetone extract of, erodium glycophyllum has high antibacterial activity against pathogenic Staphylococcus aureus. Hot Soxhlet extraction method is recommended as well as 96 well treys microtitre plate method. The MIC of tested Erodium glycobhyllum against Ttaphyllococcus aureus was 25 and 50 mg/ml, respectively. Peganum showed moderate response to Eoridium Micrococcus luteaus showed height moderate response to Erodium, However the exception of Erodium in high concentration, none of the tested plants showed any signs of toxicity in rats tested. The former produced liver fatty changes and haemorrhages in the kidneys. Toxicity experiments of Erodium glycophyllum, and Artimisa herba using experimental animals (rats) confirm the safety of such plants for therapy of various diseases. An antibacterial evolution for the total extract of tested medicinal plants could also reveal a more potent activity toward tested pathogenic Gram positive and Gram negative bacteria. However, the plants differed significantly in the activity towards tested pathogenic bacteria. The differences could be attributed to their structural nature
References
Auzi , A . ( 1999 ) : Medicinal plants in Libya . First Conferences on Natural Resources in Sert , Libya .
Faraj , I . ; Missaoui , M . ; Bougrine , H . ; Jebriel , A . and Michri , M . ( 1988 ) : Evaluation study of Economic value of forest tree in Libya .
Al-Idrissi , M . ; Sbeita , A . Jebriel , A . ; Zintani , A . ; Shreidi , A . ; Ghawawi , H . and Tazi , M . ( 1996 ) : Country Report to the FAO international Technical Conf . on Plant Genetic Resources , Leipzing , Germany .
Somanapan , A . (1990) : Medicine from herbal plant . 1st ed. Faculty of Pharmacy , Mahidol University , Bangkok , Thailand . Staphylococcus aureus infection . N . Engl . J . Med . , 340 : 517 – 523 .
Vachirasup , T . ( 1995 ) : Senna plant in Thailand . 1st ed. Faculty of Pharmacy , Mahidol University , Bangkok , Thailand . Warren E . Lewinson & Ermest Jawatz , chapter 10 . Medical Microbiology & Immunology , 3rd ed. Appleton & Lange , Norwaik , Connecticut , 42 – 52 .
Boonyaprapatsara , N . ( 2000 ) : Thai traditional Herbal Medicine plant . Vol . 4 Prachachon Publ . Bangkok , Thailand .
Grieve , H . (1981): The herb : New plants http : www : Botanical . com Botanical . mgmh comidx . Html .
Proestos , C . ; Boziaris , I . S . ; Nychas , G .J . E . and Komaitis , M . (2006) : Analysis of flavenoids and phenolic acids in Greek aromatic plants , Investigation of their antioxidant capacity and antimicrobial activity . Food Chem . 95 : 664 – 671
Oussalah , M . ; Caillet , S . ; Saucier , L . and Lacroix , M . (2006) : Antimicrobial effects of selected plant essential oils on the growth of Pseudomonas putida strain isolated from meat . Meat Science , 73 : 236 – 244
Van Vuuren , S . F . ; Viljoen , A . M . ; Van zyl , R . L .; Van Heerden F. R . ; Husnu , K . and Basser , C . ( 2006 ) : The antimicrobial , antimalarial and toxicity profiles of helihuinulone , leaf essential oil and extracts of Helichrysum cymosum L. South Africa of Botany , 72 : 287 – 290 .
Conner , D . E . and Beuchat , L . R . ( 1984 ) : Effects of essential oils from plants on growth of food spoilage Yeast . J . of food Science , 49 : 429 – 434 .
Zaika , L . L . (1988 ) : Spices and Herbs : Their antimicrobial activity and its determination
. J . of food safety , 9 : 97 – 119 .
Conner , D . E . ( 1993 ) : Naturally occurring compounds . In : P . Davidson and A . L . Branen ( eds. ) . antimicrobials in foods ( pp . 441 – 468 ) New York , N Y : Moreel Dekker
.
Dorman , H . J . and Deans , S . G . ( 2000 ) : antimicrobial agents from plants : Antimicrobial activity of plant volatile oils . J . Appl . Microbiol . 88 : 308 – 316 .
Bagamboula , C . F . ; Uyttendaele , M . and Debevere , J . ( 2003 ) : Antimicrobial effect of spices and herbs on Shigella sonnei and Shigella flexneri . J . of food protection , 66 : 668 – 673 .
Mouuia , O . ; Stephane , C . ; linda , S . and Monique , L . (2006) : Inhibitory effects of selected plant essential oils on the growth of 4 pathogenic bacteria : E . Coli , O157 : H7 , Salmonella typhimurium , Staphylococcus aureus and Listeria monocytogenes . Food control , www . Elsevier . Com Locate food count .
Bogdadi ,H. A. A.; Kokoska, L. ; Havlik, J.; kloucek , P. ; Rada , V. and Vorisek, K. ( 2007 ) : In vitro antimicrobial activity of some Libyan medicinal plant extracts . Pharmaceutical Biology , 45 ( 5 ) : 386 – 391 .
Abu-Irmailehand , B. and Afifi , F. ( 2003 ) : Herbal medicine in Jordan with special emphasis on commonly used herbs J. Ethnopharmacol. ( 89 ) : 193 – 197
Oran , S. A. and Al-Eisawi , D. M. (1998) : Check-list of medicinal plants in Jordan
.Dirasat Med. Biol. Sci. , 25 ( 2 ) : 84 – 112 .
Griffin , S . G . ; Wyllie , G . ; Markham , J . L . and Leach , D . N . ( 1999 ) : The role of structure and molecular properties of terpenoids in determining their antimicrobial activity
. Flav . fragr . J . 14 : 322 – 332 .
Rios , T. ; Recio , M. and Villar , A. (1990) : Antibacterial activity of Helichrysum stoechas
. Planta Med. 56 : 446 .
Janssen , A . M . ; Scheffer , J . J . C . ; and Svendsen , A . B . (1987) : Antimicrobial activity of essential oils : a 1976 - 1986 literature review . Aspects of the test methods . Planta Med . 5 : 395 – 398 .
Kreshaw , L . (2000) : Edible and medicinal plants of the Rockies . Lone Pine Edmonton . Canada .
Lutz , D . L .; Alviano, D . S.; Alviano, C. S. and Kolodziejczyk, P. P. (2008): Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils . Phytochemistry 69 : 1732 – 1738 .
Hudaib , M. M .and Aburjai , T. A. ( 2006) : composition of the essential oil from Artemisia herba-alba grown in Jordan . J. of Essential oil Res. , ( JEOR ) pp. 18 – 23
Zeggwagh , N. A.; Farid , O. , Michel , J. B. and Eddouks , M. (2008) : Cardio-vascular effect of Artemisia herba-alba aqueous extract in spontaneously hypertensive rats . Methods find Exp. Clin. Pharmacol. 30 ( 5 ) : 375 – 381 .
Ben Abid , Z. ; Feki , M. ; Hedhili , A. and Hamdaouil ,M. H. ( 2007 ) : J. of Nutrition , Metabolic Diseases and Dietetics 51 ( 3 ) : 16 .
Zhang , Y.Y; Li, S.H. and Tian, Z.( 1995 ) : Morphological and Histological studies of the Chinese drug Lao-guan-cao . Yao xue Bao 3 : 46 – 58 .
Hussein, F. T.K. ( 1985 ) : Medicinal plants in Libya . 1st ed. Arab Encyclopedia House, Beirut, Lebanon. P. 436 .
Gohar, A. A.; Lahloub, M.F. and Niwa, M. (2003): Antibacterial polyphenol from Erodium glaucophyllum. Z. Naturforsch. 55 : 670 – 674 .
Stock , I. ; Burak, S. ; Jane, K.; Gruger, T. and Wiedemann, B.(2003B) : Natural Antimicrobial Susceptibilities of strains of “unusual” Serratia species : S.ficaria, S.fonticola, S. odorifera, S.plymuthica and S. rubideae . J. Antimicrobial Chemotherapy 51
: 865 – 885 .
Jafri , S. and El-Gadi , A. (1978) : Asteraceae . In flora of Libya , 107. Al-Fateh University Press , Tripoli, Libya .
Perez, C.; Paul, M. and Bazerque, P. (1990): An antibiotic assay by agar well diffusion method. Acta Biol. Exper. 15:113-115.
Valgas, C. ; Machado de Sousa, S. ; Smania, FA. E. and Arthur Smania, Jr. A. Jr. (2007) : Screening methods to determine antibacterial activity of natural products. Brazillian Journal of Microbiology 38: 369-380.
Khond, M. ; Bhosale, J.D.; Arif, T.; Mandal, T.K.; Padhi, M.M. and Dabur, R. ( 2009) : Screening of some selected medicinal plant extracts for In-vitro Antimicrobial activity. Middle-East J. Scientific Res. 494) : 271-278.
Dababneh, B.F. ( 2008 ) : Antimicrobial activity of selected Jordanian medicinal plant extracts against pathogenic microorganisms. J. Food Agric. And Environm. 6(2) : 134-139.
Gibbs , R.D. (1974) : Chemotaxonomy of flowering plants . Vol. III.Mc Gill Queen,s University Press, London. pp. 1337 – 1338 .
Fecka, I. ; Kowalczyk, A. and Cisowski, W. (2001) : Phenolic acids and Depsides from some species of the Erodium genera. Z. Naturforsch., 56c : 943-950.
Kumari, Y. ; Okuda, H. ; Okuda, T. and Arichi, S. (1986) : Studies on the activities of tannins