Solid-Phase Extraction of Biogenic Amines using Sorbent Material Immobilized with Salicylaldehyde 2,4- Dinitrophenylhydrazone
Keywords:
Sol-gel, hydrazones, biogenic amines, SPEAbstract
Sorbent material based on a novel hydrazone compound, C13H10N4O5, was prepared by immobilizing the ligand into gel matrix (SG). Characterization of SG by FTIR, and solid UV-vis proved the presence of the hydrazone in the sol-gel silica. The competitive sorption characteristics of eight biogenic amines (BAs) using batch method was studied using HPLC. The extraction was optimized for key parameters such as pH, contact time, BAs concentration, ligand concentration and selectivity among the studied species. The results show that the sorbent material has special selectivity towards SPD > PUT > CAD.
References
A.R. Shalaby, Food Res. Int. 29 (1996) 675.
M.H. Silla Santos, Int. J. Food Microbiology 29 (1996) 213.
M. Křížek, T. Pelikanová, J. Chromatogr. A 815 (1998) 243.
N. Nishibori, S. Fujihara, T. Akatuki, Food Chem. 100 (2007) 491-497.
Paproski, R. E., Roy, K. I., & Lucy, C. A. (2002). Selective fluorometric detection of polyamines using micellarelectrokinetic chromatography with laser-induced fluorescence detection. Journal of Chromatography A, 946, 265-273.
Bouchereau, A., Guénot, P., &Larher, F. (2000). Analysis of amines in plant materials. Journal of Chromatography B: Biomedical Sciences and Applications,747, 49-67.
AbdassalamAbdelhafizTameem, AbdussalamSalhin, BahruddinSaad, Ismail Ab. Rahman, Muhammad IdirisSaleh, Shea-lin Ng and Hoong-Kun Fun. Benzophenone–2, 4−dinitrophenylhydrazone, ActaCryst. E(2006) E62, o5686-o5688.
AbdassalamAbdelhafiz. Tameem, B. Saad, A. Makahleh, A. Salhin, M. I. Saleh,Talanta 82(2010) 1385.
ChanbashaBasheer, Weishan Wong, Ahmad Makahleh, AbdassalamAbdelhafizTameem, AbdussalamSalhin, BahruddinSaad, and HianKee Lee, Journal of Chromatography A.1218(2011) 4332-4339.
Molday, R. S. and Kallen, R. G. (1972). Substituent effects on amide hydrogen. Journal of the American Chemical Society, 94, 6739-6745.
Gavrilenko, M. A. and Gavrilenko, N. A. (2006). Polymethacrylate sorbent for the solid-phase extraction of amines. Mendeleev Communications, 16, 117-119.
