Biocontrol Efficacy of Trichoderma harzianum and Pistacia atlantica Extract in Managing Pectobacterium carotovorum Soft Rot Disease in Potato (Solanum tuberosum L.) CV. El Mundo
DOI:
https://doi.org/10.37375/bsj.v8i21.4060Keywords:
Solanum tuberosum, Pectobacterium carotovorum, Biocontrol, Trichoderma harzianum, Pistacia atlantica, Oxidative stressAbstract
This study evaluated the synergistic efficacy of Trichoderma harzianum and Pistacia atlantica extract against Pectobacterium carotovorum-induced soft rot in potato cv. "El Mundo". Five greenhouse treatments assessed antioxidants (CAT, POD, PPO, SOD), oxidative stress markers (MDA, H₂O₂), and growth at 3 and 12 days. Combined (T4) and preventive (T5) treatments significantly mitigated oxidative stress versus infected controls (T2), with T4 recording the lowest H₂O₂ (37.27 µmol/g FW) at 3 days. Defense enzymes and protein content increased in T4 and T5. Shoot length was preserved in T4 (45.17 cm) and T5 (46.33 cm) compared to T2 (31.83 cm). Integrating these bioagents offers a sustainable soft rot management strategy via systemic resistance induction.
References
Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121–126.
https://doi.org/10.1016/S0076-6879(84)05016-0
Alfiky, A., & Weisskopf, L. (2021). Deciphering Trichoderma-plant-pathogen interactions for
better biocontrol applications. Journal of Fungi, 7(1), 61.
Bdliya, B. S., & Langerfeld, E. (2005). Soft rot and blackleg [Erwinia carotovora ssp. atroseptica
(Van Hall) Dye] of potato as affected by inoculum density and variety. Nigerian Journal of
Plant Protection, 22, 65–75.
Benhammou, N., Bekkara, F. A., & Panovska, T. K. (2018). Antioxidant activity of methanolic
extracts and some bioactive compounds of Pistacia atlantica Desf. leaves. Medicinal
Chemistry Research, 18(1), 10–15. https://doi.org/10.1007/s00044-008-9129-4
Buttimer, C., McAuliffe, O., Ross, R. P., Hill, C., O'Mahony, J., & Coffey, A. (2017).
Bacteriophages and bacterial plant diseases. Frontiers in Microbiology, 8, Article 34.
https://doi.org/10.3389/fmicb.2017.00034
Chance, B., & Maehly, A. C. (1955). Assay of catalases and peroxidases. Methods in Enzymology,
2, 764–775. https://doi.org/10.1016/S0076-6879(55)02300-8
Charkowski, A. O. (2018). The changing face of bacterial soft-rot disease. Annual Review of
Phytopathology, 56, 269–288. https://doi.org/10.1146/annurev-phyto-080417-045914
Cui, W., He, P., Munir, S., He, Y., Li, X., & Wu, Y. (2019). Biocontrol of soft rot of Chinese
cabbage using an endophytic bacterial strain. Frontiers in Microbiology, 10, Article 1971.
https://doi.org/10.3389/fmicb.2019.01971
Elsherbiny, E. A., El-Gamal, N. G., & Ghoneem, K. M. (2021). Trichoderma asperellum and
salicylic acid triggered the antioxidant defense system in onion plants against Sclerotium
cepivorum. Journal of Plant Pathology, 103(2), 521–532. https://doi.org/10.1007/s42161-021-
00776-8
FAO. (2022). World food and agriculture – Statistical yearbook 2022. Food and Agriculture
Organization of the United Nations. https://doi.org/10.4060/cc2068en
Ghalem, S., & Mohamed, B. (2020). Antimicrobial activity of the essential oil of Pistacia atlantica
Desf. from Algeria. Journal of Essential Oil-Bearing Plants, 23(1), 112–119.
https://doi.org/10.1080/0972060X.2020.1734567
Gill, S., Alam, S., & Kumar, R. (2023). Bacterial soft rot of potato: Epidemiology and management
strategies. Journal of Plant Diseases and Protection, 130(2), 235–248.
https://doi.org/10.1007/s41348-022-00689-2
Gouda, S., Kerry, R. G., Das, G., Paramithiotis, S., Shin, H.-S., & Patra, J. K. (2020). Revitalization
of plant growth-promoting rhizobacteria for sustainable development in agriculture.
Microbiological Research, 206, Article 126140. https://doi.org/10.1016/j.micres.2017.08.016
Gurjar, M. S., Ali, S., Akhtar, M., & Singh, K. S. (2012). Efficacy of plant extracts in plant disease
management. International Research Journal of Microbiology, 3(1), 4–23.
Malkhan Singh Gurjar, Shahid Ali, Masood Akhtar, Kangabam Suraj Singh (2012) Efficacy of
plant extracts in plant disease management. International Research Journal of Microbiology,
4-23.
Mauch-Mani, B., Baccelli, I., Luna, E., & Flors, V. (2017). Defense priming: An adaptive part of
induced resistance. Annual Review of Plant Biology, 68, 485–512.
https://doi.org/10.1146/annurev-arplant-042916-041132
Moghaddam, M. R. B., Van den Ende, W., & Trindade, L. M. (2022). Fructans and their role in
plant stress tolerance. Journal of Experimental Botany, 73(9), 2735–2752.
https://doi.org/10.1093/jxb/erac045
Perfileva, A. I., Strekalovskaya, E. I., Klushina, N. V., Gorbenko, I. V., & Krutovsky, K. V. (2025).
The causative agent of soft rot in plants, the phytopathogenic bacterium Pectobacterium
carotovorum subsp. carotovorum: A brief description and an overview of methods to control
it. Agronomy, 15(7), Article 1578. https://doi.org/10.3390/agronomy15071578
PNW Handbooks. (2024). Plant Disease Handbook: Potato - Bacterial Soft Rot. Pacific Northwest
Extension Publication. Oregon State University Extension Service.
https://pnwhandbooks.org/plantdisease/host-disease/potato-solanum-tuberosum-bacterial-
soft-rot
Rahman, M. M., Khan, M. A., & Islam, M. N. (2012). Comparative study of different solvents for
extraction of bioactive compounds from medicinal plants. Journal of Applied Pharmaceutical
Science, 2(8), 129–134. https://doi.org/10.7324/JAPS.2012.2819
Sanders, R. (2022, January 13). Copper-based chemicals may be contributing to ozone depletion.
Berkeley News. https://news.berkeley.edu/2022/01/13/copper-based-chemicals-may-be-
contributing-to-ozone-depletion/
Shen, Y., Wang, L., & Zhang, Q. (2025). Carvacrol and streptomycin in combination weaken
streptomycin resistance in Pectobacterium carotovorum subsp. carotovorum. Plants, 14(6),
Article 908. https://doi.org/10.3390/plants14060908
Sood, M., Kapoor, D., Kumar, V., Sheteiwy, M. S., Ramakrishnan, M., Landi, M., Aragão, F. A. S.,
Sharma, A. (2020). Trichoderma: The "secrets" of a multitalented biocontrol agent. Plants,
9(7), Article 862. https://doi.org/10.3390/plants9070862
Velikova, V., Yordanova, I., & Edreva, A. (2000). Oxidative stress and some antioxidant systems in
acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Science, 151(1),
59–66. https://doi.org/10.1016/S0168-9452(99)00197-1
Verhaegen, B., Van den Broeck, W., & Dewulf, J. (2024). Implementation of Regulation (EU)
2019/6 on veterinary medicinal products: Impact on antimicrobial use in agriculture. EFSA
Journal, 22(Suppl 1), e220415. https://doi.org/10.2903/j.efsa.2024.8723
Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Woo, S. L., & Lorito, M. (2018).
Trichoderma-plant-pathogen interactions. Soil Biology and Biochemistry, 40(1), 1–10.
https://doi.org/10.1016/j.soilbio.2007.07.002
Zhang, Y., Li, Y., Ge, Y., & Li, W. (2020). Antibacterial activity and mechanism of action of
chlorogenic acid against Pectobacterium carotovorum subsp. carotovorum. Food Control, 108,
Article 106863. https://doi.org/10.1016/j.foodcont.2019.106863








