Various technique of cell culture and slide preparation for human chromosome
DOI:
https://doi.org/10.37375/sjms.v1i2.323Keywords:
various technique, cell culture, human chromosome, hot plateAbstract
The cell culture and slide preparation method of human chromosome is the mechanism that is a corresponding change in DNA content. In this research we used various techniques of cell culture and slide preparation for human chromosome and examined for 2 days, 3 days and 4 days cell culture because these are average days for approximately the good results and the treatment will still be good also the result has minimum bias.
The research objectives were to determine the appropriate length of cell culture needed for human chromosome with different lengths and to determine the appropriate method for staining the slide for human chromosome with different lengths.
Based on the data analysis, the result of this research shows that the appropriate length of cell culture needed for human chromosome with different lengths is 2 days of incubation. Although there were no significant differences between 2,3 and 4 days length based on statistical analysis, still there is little difference between them and the best is 2 days rather than 3 and 4 days based on the graph analysis. The appropriate method for staining the slide for human chromosome with different lengths is hot plate rather than another technique
References
Goldman, J. M., et al. "The rodent estrous cycle: characterization of vaginal cytology and its utility in toxicological studies." Birth Defects Research Part B: Developmental and Reproductive Toxicology, 2007, 80(2): 84-97.
Hamzah, A. Human chromosomes, /doc/38921503/, 2011.
Kops, G. J., et al. "Finding the middle ground: how kinetochores power chromosome congression." Cellular and molecular life sciences; 2010, 67(13): 2145-2161.
Maiato, Helder. Mitosis: wisdom, knowledge and information. Universidade do Porto Rua do Campo Alegre, 2012, 823 4150-180 Porto, Portugal.
Tsafrir, D. , Manny Bacolod, Zachariah Selvanayagam, Ilan Tsafrir, Jinru Shia, Zhaoshi Zeng, Hao Liu, Curtis Krier, Robert F. Stengel, Francis Barany, William L. Gerald, Philip B. Paty, Eytan Domany,1 and Daniel A. Notterman. Relationship of Gene Expression and Chromosomal Abnormalities in Colorectal Cancer. Cancer Res 2006; 66: (4). February 15.
Xiang, Yu. and Yang , Zheng-Wei. Detachment of methacrylate-embedded sections from microscope slides can be prevented by heating on hotplate. Journal of Histology & Histopathology 2014,
Barch, M.J., Turid Knutsen, and Jack L. “The AGT Cytogenetics Laboratory Manual”, Third Edition, The Association of Genetic Technologists, Lippincott-Raven Publishers, 2015.
