Evaluation Of Hepatoprotective Activity Of Aqueous Extract Of Indian Costus Roots On Paracetamol Induced Hepatotoxicity In Albino Mice
Abstract
Indian Costus (Zingiberaceae) is widely employed in various traditional medicines. The present study is aimed at evaluating the hepatoprotective effect of aqueous extract of indian costus roots by paracetamol-induced liver damage in mice. The experimental mice were separated into five groups (n = 7 mice): group-A which kept as normal control group; group-B was treated with paracetamol (14 mg/kg b.w.) as therapeutic doses once a day intraperitoneal (IP) for four days ; group-C was treated with paracetamol (85 mg/kg b.w.) as overdoses once a day by IP administration for four days; ; groups-D and E received orally (10 µl) 10% Indian costus roots extract every day for eight days. On the fifth day , the animals of groups -D and E were treated with paracetamol (14 and 85 mg/kg b.w.) once a day for four days followed by IP administration. At the end of the experimental period, blood was obtained from each maus for the determination of serum levels of aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP). Biochemical analysis of the serum obtained showed a significant increase in the levels of AST, ALT and ALP in mice administered with14 mg/kg b.w. and 85 mg/kg b.w of paracetamol. Pre-treatment of the animals with the extract caused a decrease in the levels of these enzymes. In parallel, the histopathological assessments of the liver sections of mice also proved that extract markedly minimized the PA toxicity and maintained the liver tissues.This study has shown that the aqueous extract of indian costus roots a possesses slight hepatoprotective property.
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