Serologic survey of leishmaniasis in Ibn Sina patients, Sirte, Libya

  • Shaban R. A. Bagar Bagar Department of Medical Microbiology & Immunology, Faculty of Medicine, Sirte University, Sirte, Libya.
  • Mohammed A. Daw 1,2Department of family medicine and community health, Faculty of Medicine, Sirte University, Sirte, Libya.
  • Fathi.s.Elkasah Dermatology Department
  • Abdalla etbiga 4Department surgery, Faculty of Medicine, Sirte University, Sirte, Libya
Keywords: leishmaniasis, ELISA, , Cutaneous Leishmaniasis


The clinical and parasitological features of leishmaniasis were investigated, retrospectively, in 162 patients who attended the out-patient clinic of Ibn Sina hospital during January 2017 and June 2018. The aim was to evaluate the epidemiological characteristics of leishmaniasis. Sera from all the suspected patients were examined for slit and smear examination.

 Seventy one represent (43.82%) of 162 were positive. Of them, 52 (73%) were males and 19 (26.76%) females. The most affected age group was found to be between 21 and 30; the least affected was age group below 10 years.


El-Hassan AM, Zijlstra EE. (2001) Leishmaniasis in Sudan. Mucosal leishmaniasis. V Trans R Soc Trop Med Hyg; 95 (Suppl 1).
Hoogstraal H and Heyneman D (1969). Leishmaniasis in the Sudan Republic. 30. Final epidemiological report. Am J Trop Med Hyg.; 18:1091-1210.
Ashford RW. and Bettini S. (1987). Ecology and epidemiology: Old World. In The leishmaniases in biology and medicine, W. Peters and R.Killick-Kendrick (eds.). Academic Press, London, England,; 392-405.
Ribera, E., Ocan˜ a, I., Otero, J., Corte´s, E., Gasser, I. & Pahissa, A. (1996). Prophylaxis of visceral leishmaniasis in human immunodeficiency virus-infected patients. American Journal of Medicine, 100, 526–501.
Desjeux, P. Leishmania/HIV co-infection, southwestern Europe. (1999). Weekly Epidemiological Record. 44: 365-375.
Grimaldi Jr G, Tesh R. (1993). Leishmaniasis of the New World: currents concepts and implications for future research. Clin.Microbiol Rev 3: 230–250.
Herwaldt BL. (1999). Leishmaniasis. Lancet. 354:1191-9.
Rajasekariah GH, Ryan JR, Hillier SR, Yi LP, Stiteler JM, Cui L, et al. (2001). Optimisation of an ELISA for the serodiagnosis of visceral leishmaniasis using in vitro derived promastigote antigens. J Immunol Methods. 252: 105-19.
Jensen AT, Goafar A, Ismail A, Christensen CB, Kemp M, Hasan AH, et al. (1996). Serodiagnosis of cutaneous leishmaniasis: assessment of an enzyme linked immunosorbent assay using a peptide sequence from gene B protein. Am J Trop Med Hyg. 55:520-525.
Evans, D. A. Kinetoplastida. (1978) In Methods of cultivating parasites in vitro, A. E. R. Taylor and J. R. Baker (eds.). Academic Press, London, England. 55-58.
Pampiglione S, Manson-Bahr PEC, Giungi F, Giunti G, Parenti A & Canestri Trotti, G. (1974). Studies on Mediterranean leishmaniasis. II. Asymptomatic cases of visceral leishmaniasis. Trans R Soc Trop.Med Hyg.; 68: 447-453.
Pampiglione S, Manson-Bahr PEC, La Place M, Borgatti MA and Musumeci S. (1975). Studies in Mediterranean leishmaniasis. III. The leishmanin skin test in kala-azar. Trans R Soc Trop Med Hyg.;69: 60-68.
World Health Organization, (1990). Control of the Leishmaniasis. World Health Organ. Tech Rep Ser. 793.
Hommel MW. Peters J.R., and Quilici M. (1978). The micro-ELISA technique in the serodiagnosis of visceral leishmaniasis. Ann. of Trop. Med. Parasitol. 72: 1213-1218.
Martin SK, Thuita-Harun L, Adoyo-Adoyo M, and Wasunna KM. (1998). A diagnostic ELISA for visceral leishmaniasis, based on antigen from media conditioned by Leishmania donovani promastigotes. Ann Trop Med Parasitol.; 92:571-7.
Jensen AT, Gasim S, Moller T, Ismail A, Gaafar A, Kemp M. el Hassan AM, et al. (1999). Serodiagnosis of Leishmania donovani infections: assessment of enzyme-linked immunosorbent assays using recombinant L. donovani gene B protein (GBP) and a peptide sequence of L. donovani GBP. Trans R Soc Trop Med Hyg. 93:157-60.
Singh S, McGwire BS, and Chang KP. (1995). Kinetoplast and nuclear DNA polymorphism of visceral Leishmania isolates from North-eastern India. J Protozool Res.5:27-32.