Comparison of two genomic DNA extraction protocols from single dray seed of Barley crop

  • Ahmed Shaaban Libyan Biotechnology Research Center, Department of Tissue Culture Plant
  • Elmundr Abughnia Libyan Biotechnology Research Center, Department of Tissue Culture Plant
  • Mohammed Abosneena Libyan Biotechnology Research Center, Department of Tissue Culture Plant
  • Khaled Elhejajy Libyan Biotechnology Research Center, Department of Tissue Culture Plant
  • Kairy Elaabed Libyan Biotechnology Research Center, Department of Tissue Culture Plant
Keywords: Barley plant , plant DNA extraction , CTAB , SDS

Abstract

Modern plant breeding studies are largely based on plant genetic engineering programs . Extraction of DNA with high quality plays as a key factor in most of plant genetic studies , therefore two different DNA extraction protocols based on CTAB buffer and SDS buffer were tested  for the purpose of selecting the best DNA extraction protocol from dry seeds of nine variety of barely plant . Barley seeds were taken from Libyan seeds gene bank .After Barely samples were prepared DNA was extracted directly using CTAB and SDS solutions .The quality of extracted DNA was assessed by spectrophotometric measurements and gel electrophoresis system. The results of this study showed that purity of extracted DNA by CTAB method was clearly batter compared with SDS method . CTAB method seemed to be more effective for extracting DNA from barely dry seeds. The quality of DNA with CTAB method was good , while CTAB had overall better A260/A280 ratio (1.736-1.932) . SDS method seemed to be not suitable for DNA extraction from dry seeds of barely crop.

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Published
2022-04-17
How to Cite
[1]
Shaaban, A., Abughnia, E., Abosneena, M., Elhejajy, K. and Elaabed, K. 2022. Comparison of two genomic DNA extraction protocols from single dray seed of Barley crop. Scientific Journal for the Faculty of Science-Sirte University. 2, 1 (Apr. 2022), 126-131. DOI:https://doi.org/10.37375/sjfssu.v2i1.225.
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