Risk Factors for Ciprofloxacin and Gentamycin Resistance among Gram Positive and Gram Negative Bacteria Isolated from Community-Acquired Urinary Tract Infections in Benghazi city

  • Abdlmanam Fakron Omar Al-Mukhtar University, Faculty of Science, Department of Microbiology, Box 919, Al-Bayda – Libya
  • Noor Alawkally Department of Medical laboratory, High Institute of Science and Technology, Suluq, Libya
  • Sara El- warred Department of Statistic, Faculty of Arts and Science, University of Benghazi, Libya.
  • Mareei Aldouakali Ali Department of Medical laboratory, High Institute of Science and Technology, Suluq, Libya.
  • Mailud El-amari Department of Health Education , Faculty of Public Health, University of Benghazi, Libya
  • Alreda Awkally Central of Pharmacy, Ministry of Health, Darna, Libya
  • Mohammed Soutiyah Department of Microbiology, Faculty of Science, University of Omar Al-mukhtar, Libya
  • Nessrine Suliman Department of Zoology- Faculty of Science- University of Benghazi, Libya
  • Hamza Ibrahim Hamza Khalifa Ibrahim4Department of Pharmacy, Higher Institute of Medical Technology, Bani Waleed, Libya
Keywords: Benghazi, Ciprofloxacin, Gentamycin, E. coli, Klebseilla pneumonia, Staph aureus, Urinary Tract Infection


Urinary tract infections (UTIs) are a severe public health problem caused by a range of pathogens. The aims of the study were to investigate the prevalence of urinary tract infection and to see the pattern of Ciprofloxacin and Gentamycin susceptibility against uropathogens in Benghazi city. The study took place between 2021 April and October 2021. In-vitro antibacterial activity and resistance patterns of these two well-known antibiotics were studied and compared using the disk diffusion method. Laboratory reports and clinical data of patients with a positive urine culture (≥105 CFU/mL) were included in the study. Ciprofloxacin and gentamycin were tested against fourteen common bacterial pathogens, isolated from clinical samples of patients from Benghazi city Libya. A total of isolates were detected in 264 (75.4%) samples. Out of these, 75.4% were female and 24.6% were male. The majority of the study participants were in the age range of between 36-53 years (33.7%). The Increased bacterial resistance to ciprofloxacin has been shown. Most strains of E. coli and Staph aureus were resistant to ciprofloxacin and sensitive to gentamycin thereby showing that gentamycin is more effective than ciprofloxacin. This study showed that E. coli followed by staph aureus and K. pneumonia were the predominant uropathogen of UTIs in this geographical area. Most of the uropathogens were susceptible to Ciprofloxacin. The results showed that there is an alarming subject of resistance to Gentamicin against UTI patients in this area. Clinicians should be aware of the existing data and treat patients according to susceptibility patterns.


Abir, B. A; Hamida, E; Asma, E; Alsharif.. P. (2021). revalence and Antibiotics Susceptibility Pattern of Urine Bacterial Isolates from Tripoli Medical Center (TMC), Tripoli, Libya. IBEROAMERICAN JOURNAL OF MEDICINE 03 221-226

Al Yousef, S. A; Younis, S; Farrag, E; Moussa, H; Bayoumi, F. S; Ali, A. M. (2016). Clinical and laboratory profile of urinary tract infections associated with extended spectrum β-lactamase Producing Escherichia coli and Klebsiella pneumoniae. Ann Clin Lab Sci. 46(4):393–400.

Alanazi, M. Q; Alqahtani, F. Y; Aleanizy, F. S. (2018). An evaluation of E. coli in urinary tract infection in emergency department at KAMC in Riyadh, Saudi Arabia: retrospective study. Ann Clin Microbiol Antimicrob. 17(1):3. doi:10.1186/s12941-018-0255-z

Al-Harthi ,A. A; Al-Fifi, S. H. (2008). Antibiotic resistance pattern and empirical therapy for urinary tract infections in children. Saudi Med J. 29 (6):854–858.

Ayoyi, A. O; Kikuvi, G; Bii, C; Kariuki, S.(2017). Prevalence, aetiology and antibiotic sensitivity profile of asymptomatic bacteriuria isolates from pregnant women in selected antenatal clinic from Nairobi, Kenya. Pan Afr Med J. 26:41. doi: 10.11604/pamj.2017.26.41.10975.

Baron, E. J; Peterson, L. R; Finegold, S. M; Bailey and Scott’s. (1994). Diagnostic Microbiology. 9th ed. St Louis: Mosby; 1994:249–257.

Bauer, A. W; Kirby, W. M. M; Sherris, J. C; Turk, M. (1966). Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol. 45: 493–496.

Bitew, A; Molalign, T; Chanie, M. (2017). Species distribution and antibiotic susceptibility profile of bacterial uropathogens among patients complaining urinary tract infections. BMC Infect Dis. 17(1):654. doi:10.1186/s12879-017-2743-8

Butler, C. C; Hawking ,M. K; Quigley, A; McNulty, C. A. (2015). Incidence, severity, helps seeking, and management of uncomplicated urinary tract infection: a population-based survey. Br J Gen Pract. 65(639):e702-7. doi: 10.3399/bjgp15X686965.

Carlos, R; Kiffer,, Caio, M; Carmen, P; Oplustil, J. L; Sampaio. (2007). Antibiotic Resistance and Trend of Urinary Pathogens in General Outpatients from a Major Urban City. International Braz J Urol Vol. 33 (1): 42-49.

Cheesebrough, M. (2006). District Laboratory Practice in Tropical Countries Part II. 2nd ed. London: Cambridge University Press; 105–114.

Clinical Laboratory Standards Institute (CLSI). (2014). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI Document M100-S24. Vol. 34. Wayne, PA: Clinical and Laboratory Standards Institute; 50–57.

Das, R. N; Chandrashekhar, T. S; Joshi, H. S; Gurung, M; Shrestha, N; Shivananda, P. G. (2006). Frequency and susceptibility profile of pathogens causing urinary tract infections at a tertiary care hospital in western Nepal. Singapore Med J 47(4) : 281.

Denamur, S; Tyteca, D; Marchand, B. J; Van, B. F; Tulkens, P. M; Courtoy, P. J et al. (2011). Role of oxidative stress in lysosomal membrane permeabilization and apoptosis induced by gentamicin, an aminoglycoside antibiotic. Free Radical Biol Med. 51(9):1656-65.

Donne, C; Hershed, H; Mary, W; Carrofl, B. (2013). Microbiology Laboratory Manual Bio/2421L.

Eltahawy, A. T; Khalaf, R. M. F. (1988). Urinary tract infection at a University Hospital in Saudi Arabia: incidence, microbiology, and antimicrobial susceptibility. Ann Saudi Med. 8(4):261–266. doi:10.5144/ 0256-4947.1988.261.

Falagas, M. E; Kastoris, A. C; Kapaskelis, A. M; Karageorgopoulos, D. E. (2010). Fosfomycin for the treatment of multidrug-resistant, including extendedspectrum beta-lactamase producing, Enterobacteriaceae infections, a systematic review. Lancet Infect. Dis. 10:43–50.


Foxman, B. (2014). Urinary tract infection syndromes: occurrence, recurrence, bacteriology, risk factors, and disease burden. Infect Dis Clin North Am. 28(1):1-13. doi: 10.1016/j.idc.2013.09.003

Gilbert, J. A; Blaser, M. J; Caporaso, J. G; Jansson, J. K; Lynch, S. V; Knight, R. (2018). Current understanding of the human microbiome. Nat Med. 24(4):392- 400. doi: 10.1038/nm.4517.

Guermazi, T. S; Boujlel, S; Assoudi, M; Issaoui, R; Tlili, S; Hlaiem, M. E. (2018). Susceptibility profiles of bacteria causing urinary tract infections in Southern Tunisia. J Glob Antimicrob Resist. 12:48-52. doi: 10.1016/j.jgar.2017.09.004.

Gupta, K; Hooton. T. M; Stamm. W. E. (2001). Increasing antimicrobial resistance and the management of uncomplicated community-acquired urinary tract infections. Ann Intern Med. 135:41–50.

hakur, S; Pokhrel, N; Sharma, M. (2013). Prevalence of multidrug resistant Enterobacteriaceae and extended spectrum b lactamase producing Escherichia coli in urinary tract infection. Res J Pharm Biol Chem Sci; 4(2): 1615-1624.

Hryniewicz, K; Szczypa, K; Sulikowska, A et al. (2001). Antibioticsusceptibility of bacterial strains isolated from urinary tract infections in Poland. J Antimicrob Chemother. 47: 773–80

Hummers, P. E; Koch, M; Ohse, A. M; Heizmann, W. R; Kochen, M. M. (2005). Antibiotic resistance of urinary pathogens in female general practice patients. Scand J Infect Dis. 37: 256-61.

Hummers, P. E; Ohse, A. M; Koch, M. Heizmann, W. R; Kochen, M. M. (2005). Management of urinary tract infections in female general practice patients. Fam Pract. 22(1):71–77. doi:10.1093/ fampra/cmh720.

Jeena, P; Coovadia, H, et al. (1995). A prospective study of bacteriuria and pyuria in catheter specimens from hospitalized children, Durban, South Africa. Annals of Tropical Paediatrics 15: 153-158.

Jeena, P; Coovadia, H, et al. (1996). Bacteriuria in children attending a primary health care clinic: a prospective study of catheter stream urine samples. Annals of Tropical Paediatrics 16: 293-298.

Kala, U and Jacobs, W. (1992). Evaluation of urinary tract infection in malnourished black children. Annals of Tropical Paediatrics 12: 75-81.

Khalifa, S. G; Amal, R; Khaled, T; Abdulaziz, Z and Ezzedin, F. (1993). Antimicrobial resistance in Libya: 19702011. ISSN: 2820 (Print) 1819-6357.


Laupland, K; Ross. T; Pitout .J; Church. D; Gregson. D. (2007). Community-onset urinary tract infections: a population-based assessment. Infection. 35:150–3.

Mcquiston, H. J; Rosborg, D. M; Sternhagen, N. A. B; Llor, C; Bjerrum, L. (2013). Different recommendations for empiric first-choice antibiotic treatment of uncomplicated urinary tract infections in Europe. Scand J Prim Health. 31:235–40

Mihankhah, A; Khoshbakht, R; Raeisi, M; Raeisi, V. (2017). Prevalence and antibiotic resistance pattern of bacteria isolated from urinary tract infections in Northern Iran. J Res Med Sci. 22:108. doi: 10.4103/jrms.JRMS_889_16.

Mihankhah, A; Khoshbakht, R; Raeisi, M; Raeisi, V.(2017). Prevalence and antibiotic resistance pattern of bacteria isolated from urinary tract infections in Northern Iran. J Res Med Sci. 22:108. doi: 10.4103/jrms.JRMS_889_16.

Mohammed, M. A; Alnour, T. M; Shakurfo, O. M; Aburass, M. M. (2016). Prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in Messalata Central Hospital, Libya. Asian Pac J Trop Med. 9(8):771-6. doi: 10.1016/j.apjtm.2016.06.011.

Mostafa, M. M; Albakosh, A. M; Alrtail, A; Rzeg, M. M and Aboukay,A. M. (2016). Etiology of uropathogenic bacteria in patients with urinary tract infection in Zliten, Libya. J Human Applied Sci.;29:16-32.

Nili, H & Asasi, K. (2002). Natural cases and an experimental study of H9N2 avian influenza in commercial broiler chickens of Iran. Avian Pathology, 31(3), 247-252

How to Cite
Fakron, A., Alawkally, N., El- warred, S., Aldouakali Ali , M., El-amari , M., Awkally, A., Soutiyah, M., Suliman, N. and Ibrahim, H. 2022. Risk Factors for Ciprofloxacin and Gentamycin Resistance among Gram Positive and Gram Negative Bacteria Isolated from Community-Acquired Urinary Tract Infections in Benghazi city. Scientific Journal for the Faculty of Science-Sirte University. 2, 1 (Apr. 2022), 76-87. DOI:https://doi.org/10.37375/sjfssu.v2i1.204.